Invitrogen™ Equine IL-1RA ELISA Kit

Catalog No.	Quantity
EEIL1RNX10	10 x 96 Tests

Catalog No. EEIL1RNX10 Supplier Invitrogen™ Supplier No. EEIL1RNX10

Description

ELISA

The Equine Interleukin-1 Receptor Antagonist (Eq IL-1RA) ELISA quantitates Eq IL-1RA in equine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Eq IL-1RA. Principle of the method The Equine IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Specifications

• Accession Number: O18999
• Assay Range: 10-3000 pg/mL
• Assay Sensitivity: 10 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cell Structure/Organelle Proteins
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Horse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Anakinra; CD121A; cytokine; D2S1473; DIRA; F630041P17Rik; ICIL-1RA; IL 1 receptor antagonist; IL1 inhibitor; il1 receptor; il-1 receptor; IL1 receptor antagonist; IL1F3; IL1R; il-1r; IL1RA; IL-1RA; IL1-RA; IL-1ra3; IL-1R-alpha; IL1RN; IL-1RN; IL1RN (IL1F3); interleukin 1 receptor antagonist; interleukin-1 receptor; interleukin-1 receptor antagonist; interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist secretory form; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; IRAP1; MGC10430; MVCD4; P80; type II interleukin-1 receptor antagonistAnakinra; CD121A; cytokine; D2S1473; DIRA; F630041P17Rik; ICIL-1RA; IL 1 receptor antagonist; IL1 inhibitor; il1 receptor; il-1 receptor; IL1 receptor antagonist; IL1F3; IL1R; il-1r; IL1RA; IL-1RA; IL1-RA; IL-1ra3; IL-1R-alpha; IL1RN; IL-1RN; IL1RN (IL1F3); interleukin 1 receptor antagonist; interleukin-1 receptor; interleukin-1 receptor antagonist; interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist secretory form; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; IRAP1; MGC10430; MVCD4; P80; type II interleukin-1 receptor antagonist; IL-1RA
• Gene ID (Entrez): 100034236
• Gene Symbol: IL1RN
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-1RN, IL-1ra, IRAP, IL1 inhibitor
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL1RA
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min