Invitrogen™ Mouse IL-2 Uncoated ELISA Kit

Catalog No.	Quantity
50-171-92	10 x 96 Tests
50-171-90	20 x 96 Tests

Catalog No. 50-171-92 Supplier Invitrogen™ Supplier No. 88702488

Description

ELISA

The Mouse Interleukin-2 (IL-2) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-2. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P04351
• Assay Range: 2-200 pg/mL
• Assay Sensitivity: 2 pg/mL
• Content And Storage: Capture Antibody: Pre-titrated, purified antibody
  Detection Antibody: Pre-titrated, biotin-conjugated antibody
  Standard: Recombinant protein for generating standard curve and calibrating samples
  10X Coating Buffer: Buffer for plating the Capture Antibody
  5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
  Enzyme: Pre-titrated Streptavidin-HRP
  Substrate: 1X TMB Solution
  Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
  96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Conjugate: HRP
• Immunoassay Kit Format: Uncoated ELISA Kit
• Product Type: ELISA
• Sample Type: Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGFaldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF; DIF-alpha; TNFA; TNFSF2; TNLG1F; TNF
• Gene ID (Entrez): 16183
• Gene Symbol: IL2
• Kit Contents: Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
• Label or Dye: Biotin
• Protein Aliases: IL-2, T-cell growth factor, TCGF
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 100 μL, Supernatant 100 μL
• Storage Requirements: 2-8°C
• Synonym: Interleukin-2
• Target: IL-2
• Test Time: 1 hr 45 min
• Total Assay Time: 24 hr 30 min