Invitrogen™ Mouse IL-31 ELISA Kit

Catalog No.	Quantity
50-112-5448	96 Tests

Catalog No. 50-112-5448 Supplier Invitrogen™ Supplier No. BMS6030

Description

ELISA

The Mouse Interleukin-31 (Ms IL-31) ELISA quantitates Ms IL-31 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-31. Principle of the method The Mouse IL-31 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.

Specifications

• Accession Number: Q6EAL8
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.1 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to mouse IL-31
  Biotin-Conjugate anti-mouse IL-31 monoclonal antibody
  Streptavidin-HRP
  Mouse IL-31 Standard lyophilized, 2000 pg/mL upon reconstitution
  Sample Diluent
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1700013B14Rik; IL31; IL-31; ILN; Interleukin; interleukin 31; interleukin-311700013B14Rik; IL31; IL-31; ILN; Interleukin; interleukin 31; interleukin-31; 1700013B14Rik
• Gene ID (Entrez): 76399
• Gene Symbol: Il31
• Interassay CV: 5.6%
• Intraassay CV: 7.2%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-31
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2-8°C
• Synonym: Interleukin-31
• Target: IL-31
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min