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Sino Biological Recombinant Human IL-35 Protein (Fc-Flag & Fc-His Tag)
Supplier: Sino Biological CT103H2303H50
A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 1; A DNA sequence encoding the human IL27B (NP_005746.2) (Met1-Lys229) was fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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