Invitrogen™ Human IL-5RA/CD125 ELISA Kit

Catalog No.	Quantity
EHIL5RAX10	10 x 96 Tests

Catalog No. EHIL5RAX10 Supplier Invitrogen™ Supplier No. EHIL5RAX10

Description

ELISA

The Human Interleukin 5 Receptor Alpha/CD125 (Hu IL-5RA) ELISA quantitates Hu IL-5RA in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-5RA. Principle of the method The Human IL-5RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.

Specifications

• Accession Number: Q01344
• Assay Range: 0.15-40 ng/mL
• Assay Sensitivity: 150 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CD125; CD125 antigen; CDw125; HSIL5R3; IL-5 receptor alpha chain; IL-5 receptor subunit alpha; Il5r; IL-5R subunit alpha; Il5ra; IL-5RA; IL-5R-alpha; interleukin 5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor subunit alphaCD125; CD125 antigen; CDw125; HSIL5R3; IL-5 receptor alpha chain; IL-5 receptor subunit alpha; Il5r; IL-5R subunit alpha; Il5ra; IL-5RA; IL-5R-alpha; interleukin 5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor subunit alpha; CD125; CDw125; HSIL5R3; IL5R
• Gene ID (Entrez): 3568
• Gene Symbol: IL5RA
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: IL5RA
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min