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Invitrogen™ Human IL-9 ELISA Kit

Catalog No. BMS20812
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96 Tests
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BMS20812 96 Tests
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Catalog No. BMS20812 Supplier Invitrogen™ Supplier No. BMS20812
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ELISA

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
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Specifications

Accession Number P15248
Assay Range 3,1 to 200 pg/mL
Assay Sensitivity 0.5 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3578
Gene Symbol Il9
Interassay CV 4.8%
Intraassay CV 5.3
Kit Contents Pre-coated 96 well plate, Standard (2 vials), Sample diluent, Biotinylated Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, SAV-HRP, TMB, Stop solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target IL-9
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 30 mins
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