Invitrogen™ Human IL-9 ELISA Kit

Catalog No.	Quantity
BMS20812	96 Tests

Catalog No. BMS20812 Supplier Invitrogen™ Supplier No. BMS20812

Description

ELISA

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

Specifications

• Accession Number: P15248
• Assay Range: 3.1-200 pg/mL
• Assay Sensitivity: 0.5 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40;
• Gene ID (Entrez): 3578
• Gene Symbol: Il9
• Interassay CV: 4.8%
• Intraassay CV: 5.3%
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Sample diluent, Biotinylated Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate,SAV-HRP,TMB, Stop solution, Adhesive Plate Covers, Standard (2 vials), Sample diluent, Biotinylated Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate, SAV-HRP, TMB, Stop solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Cytokine P40, homolog of mouse T cell and mast cell growth factor 40, IL-9, T-cell growth factor P40, Interleukin-9, p40 cytokine, p40 T-cell and mast cell growth factor
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: IL-9
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min