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Invitrogen™ Image-iT™ Low Density Lipoprotein Uptake Kit, Bodipy FL
Description
The kit was designed to specifically detect binding and uptake of LDL through the LDL receptor internalization pathway, allowing maximum control and flexibility in the experimental design. It can be used with fluorescence microscopy, flow cytometry, cell sorting, high-throughput analysis, and high content analysis.
• Optimized LDL uptake kit with two built-in controls
• Bodipy FL is detectable with GFP/FITC filter set (absorption peak: ∼515 nm, emission peak: ∼520 nm)
• Bodipy FL is fixable and multiplexable with orange, red, deep red, violet, and blue emission dyes, such as RFP, mCherry, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red
Two controls are included in the kit: unlabeled LDL as an optional pretreatment to occlude cell surface receptors in advance of probing with the labeled construct, and heparin which will chelate the LDL in the solution phase outside of the cells, preventing binding and uptake. When cells are labeled in the absence of these pretreatment controls, a fluorescent signal indicates specific LDL receptor-mediated endocytosis.
Specifications
Specifications
| Format | Kit |
| Quantity | 200 μL kit |
| Conjugate | Bodipy FL |
| Content And Storage | • 1 x 200 μL Bodipy FL-LDL, 1 mg/mL • 1 x 200 μL LDL (unlabeled), 2.5 mg/mL • 1 x 50 mL LDL assay buffer, 1 X • 1 vial heparin, 5 mg/mL • 1 x 150 mg BSA Store in refrigerator (2°C to 8°C) and protect from light. |
| Label Type | BODIPY Dyes |
| Shipping Condition | Wet Ice |
| Form | Solution |
| Protein Subtype | LDL |
| Product Line | Molecular Probes |
Frequently Asked Questions (FAQs)
We do not recommend doing this as the oxidation process (such as oxidation with copper sulphate) may result in the degradation of the BODIPY FL dye.
The LDL in BODIPY FL LDL is not acetylated.
If the BODIPY FL LDL is applied to live cells first and then fixed, the use of methanol or acetone fixation is not recommended. Exposure to alcohols, acetone, and other organic solvents can dissociate the BODIPY FL dye from the LDL.
No. Exposure to detergents and surfactants can dissociate the BODIPY FL dye from the LDL.
The BODIPY FL dye is complexed to hydrophobic domains of the LDL; it is not covalently attached.
For Research Use Only. Not for use in diagnostic procedures.
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