The IMAP TR-FRET phosphodiesterase evaluation assay kit is a homogeneous, mix-and-read biochemical assay for measuring PDE activity using IMAP progressive binding with time-resolved FRET detection. The kit supplies FAM-labeled cAMP and cGMP substrates with optimized reaction and binding buffers for high-throughput screening in 384-well format.
- Provides FAM-labeled cAMP and FAM-labeled cGMP substrates.
- Uses IMAP progressive binding and TR-FRET detection for homogeneous readouts.
- Supplies progressive binding reagent, binding buffers, TR-FRET donor, and reaction buffers.
- Configured for 20 μL reactions giving approximately 800 data points in 384-well format.
- Includes reaction buffers with BSA and with Tween-20 for assay flexibility.
- Store kit components at 4°C; aliquot substrates and store at -20°C; protect substrates from light.
- Intended for in-vitro research use only; not for diagnostic procedures.