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Thermo Scientific™ Imject™ Ovalbumin (in PBS)

Prepare hapten-carrier conjugates to generate antibodies using this conveniently formulated, purified ovalbumin (OVA) protein.

Manufacturer:  Thermo Scientific™ 77120

Catalog No. PI77120


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Description

Description

Thermo Scientific™ Imject™ Ovalbumin Carrier Protein is purified ovalbumin protein conveniently formulated for preparation of hapten-carrier conjugates.

Because ovalbumin is only weakly immunogenic, its primary use in antibody production workflows is as a secondary (independent) carrier protein to make antigens more amenable to antibody screening procedures. For example, peptide antigens are more easily coated in polystyrene microplates when conjugated to ovalbumin, thereby facilitating the screening of antisera that were produced using immunogen conjugates with KLH or other more immunogenic carrier protein.

Highlights:

  • Purified from chicken egg whites and lyophilized in phosphate buffered saline
  • Single polypeptide protein with molecular mass of 45,000 daltons
  • Contains 20 lysine residues, most of which have primary amines that are capable of reacting with a conjugation reagent
  • Contains 14 aspartic acid and 33 glutamic acid residues whose carboxyl groups account for the net negative charge (pI 4.63) of ovalbumin
  • Not as immunogenic as keyhole limpet hemocyanin (KLH) but more soluble in DMSO (dimethyl sulfoxide) for applications that require this solvent
  • Useful as an irrelevant protein carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten
Specifications

Specifications

Supplied: Carrier proteins are supplied lyophilized. When reconstituted with 2mL of ultrapure water the buffer compositions for BSA and mcKLH is 0.1M sodium phosphate, 0.15M NaCl; pH 7.2 and for OVA is 0.01M sodium phosphate, pH 7.2. Proteins contain proprietary stabilizers.

Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature
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