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Description
Includes
- IgG
- Whole serum
- Albumin
- Antibody to whole serum
- Antibody to IgG
- UltraSpec-Agarose™
- Electrophoresis buffer (25X concentrate)
- 50 Microtest tubes
- Two 10mL pipets
- 20 Filter papers
- 10 Well cutters
- 10 60mm Petri plates
Requires
- Horizontal electrophoresis apparatus
- Power supply
- Automatic micropipet and tips (5-50μL)
- Waterbath (set to 55°C)
- Microscope slides (1 x 3 in.)
- Distilled water
- 400 to 600mL Beaker
- 1000mL Graduated cylinder
- Bunsen burner, hotplate, or microwave
- Container with lid (large enough to hold electrophoresis trays)
- Paper toweling
- Plastic wrap or foil
- 37°C Incubation oven
- Lets students examine the specificity of the antigen-antibody interaction
- Identifies proteins based on their combined electrophoretic and immunological properties
- Experiment uses agarose gel electrophoresis
No experiment components contain human serum.
Specifications
Specifications
| Grade | 9 to Undergraduate |
| Includes | Instructions, proteins, antibodies; reagents, agarose, buffer, transfer pipets, well cutters, tubes, filter paper, 10 mL pipettes, and 60 mm petri plates |
| Quantity | 10 separations |
| Requires | Horizontal electrophoresis apparatus, power supply, automatic micropipettes with tips, microscope slides (1 x 3 in.), distilled water, 400-600 mL beaker, 1000 mL graduated cylinder, microwave, humidifying chamber, paper towels, plastic wrap or foil, and 37°C incubation oven (optional) |
| Time Range | Electrophoresis: 60 minutes; Incubation: overnight |
| Content And Storage | Refrigerator |
| Class Size | 10 Lab Groups |
| Product Type | Electrophoresis Kit |
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