Edvotek™ Immunoelectrophoresis Kit

Description

Includes

• IgG
• Whole serum
• Albumin
• Antibody to whole serum
• Antibody to IgG
• UltraSpec-Agarose™
• Electrophoresis buffer (25X concentrate)
• 50 Microtest tubes
• Two 10mL pipets
• 20 Filter papers
• 10 Well cutters
• 10 60mm Petri plates

Requires

• Horizontal electrophoresis apparatus
• Power supply
• Automatic micropipet and tips (5-50μL)
• Waterbath (set to 55°C)
• Microscope slides (1 x 3 in.)
• Distilled water
• 400 to 600mL Beaker
• 1000mL Graduated cylinder
• Bunsen burner, hotplate, or microwave
• Container with lid (large enough to hold electrophoresis trays)
• Paper toweling
• Plastic wrap or foil
• 37°C Incubation oven

Intermediate level experiment uses immunoelectrophoresis to separate and characterize a mixture of proteins.

• Lets students examine the specificity of the antigen-antibody interaction
• Identifies proteins based on their combined electrophoretic and immunological properties
• Experiment uses agarose gel electrophoresis

No experiment components contain human serum.

Specifications

• Product Type: Electrophoresis Kit
• Grade: 9 to Undergraduate
• Includes: Instructions, proteins, antibodies; reagents, agarose, buffer, transfer pipets, well cutters, tubes, filter paper, 10 mL pipettes, and 60 mm petri plates
• Quantity: 10 separations
• Requires: Horizontal electrophoresis apparatus, power supply, automatic micropipettes with tips, microscope slides (1 x 3 in.), distilled water, 400-600 mL beaker, 1000 mL graduated cylinder, microwave, humidifying chamber, paper towels, plastic wrap or foil, and 37°C incubation oven (optional)
• Time Range: Electrophoresis: 60 minutes; Incubation: overnight
• Content And Storage: Refrigerator
• Class Size: 10 Lab Groups