Edvotek™ In Search of the Cholesterol Gene

Description

Includes

instructions, cholesterol standard solution, standard DNA markers, control samples, simulated patient serum samples and DNA samples, cholesterol oxidase enzyme, potassium iodide, color enhancer & color developer, agarose, electrophoresis buffer, InstaStain™ Ethidium Bromide

Requires

• Horizontal gel electrophoresis apparatus
• Hotplate, micropipets, balance, waterbath, and spectrophotometer
• 250mL Flasks
• UV transilluminator
• All necessary safety equipment
• Distilled or deionized water

This experiment includes reagents for the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students will analyze a simulated genetic screening for a disease.

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor for both conditions. The genetic disease familial hypercholesterolemia (FH) causes an increase in blood levels of the 'bad' form of cholesterol, low-density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment includes reagents for the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students will analyze a simulated genetic screening for a disease.

Specifications

• Product Type: Electrophoresis Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Agarose gel electrophoresis
• Includes: Instructions, cholesterol standard solution, standard DNA markers, control samples, simulated patient serum samples and DNA samples, cholesterol oxidase enzyme, potassium iodide, color enhancer and color developer, agarose, electrophoresis buffer, InstaStain™ ethidium bromide
• Requires: Horizontal gel electrophoresis apparatus, power supply, automatic micropipette with tips, balance, microwave oven or hot plate, incubation oven or water bath, spectrophotometer and cuvettes, UV transilluminator, pipet pumps or bulbs, lab glassware, large weigh boats
• Time Range: Complete in 2 hours 15 minutes
• Content And Storage: Some components require freezer and refrigerator storage
• Class Size: 10 Lab Groups