Edvotek™ In Search of the Kissing Disease

Description

Includes

instructions, samples, antigens; antibodies, various solutions and reagents, pipets and microtest tubes

Infectious mononucleosis is common among young people of high school and college age.

• The causative agent is Epstein-Barr virus (EBV) which can be passed on through saliva during kissing
• Search for the presence of EBV using the ELISA reaction to detect specific viral proteins
• No human or viral materials used in this experiment

Specifications

• Grade: 9 to Undergraduate
• For Use With (Application): Perform an enzyme-linked immunosorbent assay (ELISA) in order to screen simulated serum samples for antibodies to the Epstein Barr Virus (EBV)
• Includes: Instructions, 10X ELISA wash buffer, ELISA dilution buffer, antigen (lyophilized), primary antibody (lyophilized), secondary antibody (lyophilized), ABTS substrate (lyophilized), and ABTS reaction buffer, small transfer pipets, microplate, 15 mL conical tubes, and snap-top microcentrifuge tubes
• Requires: Automatic micropipettes with tips (recommended), paper towels, distilled or deionized water, beakers or flasks, disposable lab gloves, and safety goggles
• Time Range: 50 min.
• Content And Storage: Refrigerator
• Class Size: 10 Lab Groups