This 96-well Human Adrenoceptor Beta 3 (ADRB3) Reporter Assay System screens test compounds by utilizing proprietary human cells. These cells constitutively express Human ADRB3, featuring a luciferase reporter gene linked to cyclic AMP Response Elements. The system quantifies luciferase activity to measure drug-induced ADRB3 changes, allowing assessment of activating or inhibitory functional activities.
- Utilizes human cells engineered for ADRB3 expression.
- Measures ADRB3 activity via a luciferase reporter gene linked to CRE.
- Cryo-preserved reporter cells for immediate dispensing.
- All-inclusive kit contains cells, media, activator, detection reagents, and assay plate.
- Offers high sensitivity with broad linear dynamic range.
- Provides stable light emission for 5 to 90+ minutes.
- Facilitates antagonist-mode assay setup with Isoproterenol.