The H4N6 (A/mallard/Ohio/657/2002) HA ELISA Pair Set is for the quantitative determination of H4N6 (A/mallard/Ohio/657/2002) HA.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H4N6 (A/mallard/Ohio/657/2002) HA coated on a 96-well plate. Standards and samples are added to the wells, and any H4N6 (A/mallard/Ohio/657/2002) HA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-H4N6 (A/mallard/Ohio/657/2002) HA monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of H4N6 (A/mallard/Ohio/657/2002) HA present in the sample. To end the enzyme reaction, the stop solution is added and abso