Innovating Science™ AP Biology Investigation Kit No. 8: Bacterial Transformation

Description

Includes

• Sterile Calcium Chloride 0.05M (30mL)
• 16 Sterile volumetric pipets
• Eight Pipet bulbs
• Eight micropipets w/plunger (10μL)
• 16 Microcentrifuge Tubes (1.5mL)
• Eight sterile inoculating loops
• 40 Petri dishes
• Four Luria agar (200mL)
• One Luria broth tube (9mL)

Perishable materials are supplied via coupon redeemable by fax, phone or e-mail, and include:

• One E. Coli culture (freeze dried)
• Two bottles 0.02g Ampicillin
• 1000ng pUC 19 Plasmid
• One Tryptic Soy Broth tube (9mL)
• One Tryptic Soy Agar slant (6mL)
• One sterile, disposable pipet

Students genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance.

 

• Efficiency of the procedure is determined by examining the number of resistant bacteria
• Includes teachers manual and student study guide copymasters
• Meets AP Science Practices 1, 3, 5, 6, and 7, and Big Idea 3
• For eight lab groups

 

Recommended: Incubator (37°C). Requires: Large beaker, microwave (not necessary if melting agar in a waterbath), microfuge tube racks, hot plate or waterbath, markers, and container of ice.

Specifications

• Certifications/Compliance: Meets AP science practices 1, 3, 5, 6, and 7 and Big Idea 3
• Product Type: AP™ Biology Investigation Kit #8: Biotechnology: Bacterial Transformation
• Color: White
• Grade: 7 to 14
• Age: 13 to 20
• For Use With (Application): Students genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance
• Includes: 30ml 0.05m sterile calcium chloride, 16 sterile volumetric pipets, 8 pipet bulbs, 8 x 10μl micropipets with plunger, 16 x 1.5ml microcentrifuge tubes, 8 sterile inoculating loops, 40 petri dishes, 4 x 200ml luria agar, 9ml luria broth tube and coupon for perishable supplies
• Quantity: 1
• Dimensions (L x W x H): 12.125 x 9.25 x 6.5 in.
• Class Size: 8 Lab Groups