Innovating Science™ Small Group Learning: Chromatography Of Amino Acids

Description

In this activity, students will perform a paper chromatography experiment on three known amino acids, and then use their results to identify the components of an unknown amino acid mixture.

• Amino acids are the fundamental building blocks of all proteins.
• Often times scientists may need to identify the amino acid contained in a protein.
• Paper chromatography is one method for separating and identifying individual amino acids.
• This Chromatography of Amino Acids activity, students will perform a paper chromatography experiment on three known amino acids, and then use their results to identify the components of an unknown amino acid mixture
• Kit contains enough material for 15 groups
• Teacher Manual and Student Study Guide copymasters are included
• Kit Includes:
  • 95% Ethanol Solution (4 x 25 mL)
  • Chromatography Solvent (4 x 25 mL)
  • Alanine Control (30 mL)
  • Glycine Control (30 mL)
  • Leucine Control (30 mL)
  • Unknown Amino Acid Mixture (30 mL)
  • 1 bottle Ninhydrin Powder
  • 8 Capillary Tubes
  • 20 Chromatography Papertes

Specifications

• Certifications/Compliance: Next Gen Aligned
• Product Type: Chromatography Kit
• Color: White
• Grade: 7 to 14
• Age: 13 to 20
• For Use With (Application): Chemistry
• Includes: 0.5 g Ninhydrin Powder, 4 x 25 mL 95% Ethanol Solution, 2 x 25 mL Chromatography Solvent, 10 mL Alanine Control, 10 mL Glycine Control, 10 mL Leucine, Control, 10 mL Unknown Amino Acid Mixture, 4 Capillary Tubes, 1 Centrifuge Tube, 7 Chromatography Sheets
• Material: Cardboard Box Kit
• Quantity: 1
• Dimensions (L x W x H): 9 x 6 x 4 in.
• Class Size: 5 Lab Groups

Next Generation Science Standards is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards was involved in the production of, and does not endorse, this product.