Instrument Control Terbium TR-FRET Kit

Catalog No.	Quantity
A14138	1 mL kit

Catalog No. A14138 Supplier LIFE TECHNOLOGIES Supplier No. A14138

Description

Instrument Control Terbium TR-FRET Kit

The Instrument Control Terbium TR-FRET Kit allows for the rapid assessment of instrument setup for terbium (Tb)-based TR-FRET assays. LanthaScreen™ and Premo™ assays require specific instrument settings. Proper instrument setup is critical to experimental success. This kit contains one tube each of 1x high and low controls which may also be used as positive control samples when running Tb TR-FRET assays.

The Instrument Control Terbium TR-FRET Kit Allows You to:

Set up instruments for Tb TR-FRET assays quickly

Check instrument Tb TR-FRET settings

Read a high and low Tb TR-FRET signal control on each assay plate

Determine if Your Instrument is Set Up to Read Tb TR-FRET Assays
Setting up your instrument to read Tb TR-FRET is the first step in running a successful Tb TR-FRET assay. This kit allows you to quickly determine if your instrument is set up properly to read Tb TR-FRET assays. Simply add a small volume into separate wells, place the plate into the instrument and read. If the ratio of high to low control signal is below 2 the instrument may not be setup properly. If the ratio of high to low control signal is between 2 and 4 you can proceed with a Tb TR-FRET.

Use the Kit as a Positive Control in Tb TR-FRET Assays
When running Tb TR-FRET assays, add a small volume of the high and low controls to separate wells, on your assay plate. Place the plate into the instrument and read, according to instructions. A high to low control signal ratio of between 2 and 4 offers you the confidence that the instrument is performing well.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Specifications

• Product Line: LanthaScreen
• Shipping Condition: Wet Ice
• Color: Green
• For Use With (Equipment): HTS Reader, Microplate Reader
• Quantity: 1 mL kit

Frequently Asked Questions (FAQs)

I have no assay window in my TR-FRET assay. What is the reason?

The single most common reason that a TR-FRET assay fails is that an incorrect choice of emission filters was made. Unlike other fluorescent assays, the filters used in a TR-FRET assay must be exactly those that are recommended for your instrument as the emission filter choice can make or break the assay. The excitation filter has more of an impact on the assay window. Please refer to our instrument setup guides in our instrument compatibility portal (https://www.thermofisher.com/instrumentsetup). If your instrument is not listed there, please contact Drug Discovery Technical Support at drugdiscoverytech@thermofisher.com.

Note: Test your microplate reader's TR-FRET setup before you begin any work with your assay. Using reagents that you have already purchased for your assay, you can test the microplate reader's TR-FRET setup. Please refer to the Terbium (Tb) Assay (http://tools.thermofisher.com/content/sfs/manuals/LanthaScreen_Tb_Instrument_Control_Application_Note17Sep10.pdf) and Europium (Eu) Assay (http://tools.thermofisher.com/content/sfs/manuals/Eu_Instrument_Control_AppNote_12Nov10.pdf) Application Notes for plate reader setup.

For Research Use Only. Not for use in diagnostic procedures.