Intact Genomics (IG®) C43(DE3) Chemically Competent Cells are optimized for toxic and difficult protein expression using IPTG-inducible T7 promoter vectors. This BL21(DE3) mutant strain was selected from C41(DE3) survivor cells that overexpressed toxic subunit b of E. coli F-ATPase. C43(DE3) has mutations to prevent cell death during recombinant protein expression and effectively expresses toxic proteins from diverse organisms. Performance is patent-validated and supported by numerous publications.
- Optimized for toxic and difficult protein expression.
- Mutant BL21(DE3) derivative with improved protein yield.
- Mutations prevent cell death during recombinant protein expression.
- Effective for expressing toxic proteins from diverse organisms.
- Transformation efficiency ≥ 3.0 x 107 cfu/μg pUC19 DNA.
- Shipped on dry ice.