IG™ Autoinduction DE3 ElectroCompetent cells are suitable for transformation and routine recombinant protein expression from an IPTG-inducible T7 promoter vector. This Autoinduction strain features a chromosomally integrated IPTG-inducible promoter, enabling T7 RNA polymerase expression via IPTG or optimized autoinduction media. They offer simple, less hands-on autoinduction, potentially boosting yields of expressed cells and protein 5 to 10 fold for various protein targets.
- Suitable for transformation and routine recombinant protein expression from an IPTG-inducible T7 promoter vector.
- Simple, less hands-on autoinduction.
- Yields of expressed cells and protein are likely 5 to 10 fold or more for a broad range of protein targets.
- T7 expression strain optimized for autoinduction.
- Suitable for expression of toxic genes.
- Tetracycline resistant (12.5 μg/mL).