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INTACT GENOMICS INC T7 Endonuclease I, 1,250 Units
Supplier: INTACT GENOMICS INC 3612
T7 Endonuclease I is a stable homodimer of identical 149 amino acid subunits, produced from a recombinant gene in *E. coli*. It recognizes and cleaves mismatched DNA where the mismatched DNA length position is more than 1 bp. This enzyme is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells, which are generated by CRISPR/ZFN/TALEN at desired target sites, PCR-amplified, denatured, and re-annealed to form mismatched DNA.
- Resolves four-way junction or branched DNA.
- Detects or cleaves heteroduplex and nicked DNA.
- Randomly cleaves linear DNA for shot-gun cloning.
- Physical purity is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining.
- Includes T7 Endonuclease I and 10x T7 Endonuclease I reaction buffer.
- Storage temperature: -20°C.
- Free from detectable contaminating nuclease activities.
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