Taq DNA Ligase provides high specificity for ligation-based PCR, SNP detection, and molecular diagnostics. This enzyme catalyzes phosphodiester bond formation in duplex DNA, using NAD+. It enables precise, high-temperature ligation (45°C to 70°C), suitable for applications requiring accuracy, including single-base substitution detection.
- High specificity for PCR, SNP detection, and molecular diagnostics.
- Enables precise, high-temperature DNA ligation (45°C to 70°C).
- Catalyzes phosphodiester bond formation in duplex DNA with NAD+ cofactor.
- Detects single-base substitutions; protein purity ≥99%.
- Free from detectable RNase and DNA endonuclease activities.
- Ideal for allele-specific gene detection (LDR, LCR) and mutagenesis.
- Includes Taq DNA Ligase and 10X Taq DNA Ligase Buffer.