Recombinant Protein;100UG;Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 23 - Ser 1122 (ITGA10) & Gln 21 - Asp 728 (ITGB1) (Accession # O75578-1 (ITGA10) & P05556-1 (ITGB1)).;Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA10 and ITGB1, has a calculated MW of 126.6 kDa (ITGA10) and 83.7 kDa (ITGB1). Subunit ITGA10 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 140-160 kDa (ITGA10) and 100-130 kDa (ITGB1) respectively due to glycosylation.;Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wa) with a linear range of 0.02-0.313 μg/mL (QC tested).