The Fish Interleukin 1B (IL-1B) ELISA Kit is designed to quantitatively measure IL1B levels in serum plasma and tissue homogenates with high precision This kit detects IL1B within a broad dynamic range of 3 12 pg/mL to 200 pg/mL offering a sensitivity as low as 0 78 pg/mL Utilizing a sandwich ELISA methodology it ensures reliable and specific detection of the target protein The assay requires a short incubation period of 1-5 hours and uses a sample volume of 50-100 L per test Results are measured at an absorbance wavelength of 450 nm providing a straightforward workflow for researchers studying immune responses or inflammatory pathways in fish models Its robust performance makes it suitable for applications in both basic research and biomarker validation