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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set

Catalog No. 501129037
Encompass_Preferred
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Quantity:
1 kit
Contains Sodium azide and formaldehyde
Contains Sodium azide and formaldehyde

Includes

  • IC Fixation Buffer
  • Permeabilization Buffer (10X)

Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal-to-noise ratios.

  • In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
  • The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
  • Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
  • Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
  • This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
  • Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
  • Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation
TRUSTED_SUSTAINABILITY

Specifications

Product Line eBioscience™
Product Type Fixn/Perm Buffer Set
Quantity 1 kit
Solution Type Fixation/Permeabilization Buffer

For Research Use Only. Not for use in diagnostic procedures.

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