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Invitrogen™ 2-YT Broth

2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors.

Supplier:  Invitrogen™ 22712020

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Catalog No. 22-712-020

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Description

Description

2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors. The 2-YT formulation is similar to Lennox LB medium, but contains double the concentration of yeast extract and a 60% higher concentration of peptone. 2-YT medium allows cells to reach higher density and increase recombinant protein production compared to LB media formulations.

2-YT Broth formulation
Formulation per liter: 16 g SELECT Peptone 140 (pancreatic digest of casein), 10 g SELECT Yeast Extract, and 5 g NaCl.
Note: To prepare 1 L of 2-YT medium, weigh 31 g of powder and add distilled water to 1 L. Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 20 minutes at 121°C.

Primary applications
• Growth of hosts for replication of M13 vectors
• Recombinant protein expression
• Achieve higher plasmid DNA yield

Find the media format that fits you needs
We offer a variety of ready-to-use media formulations including LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301) and S.O.C Recovery Medium (Cat. No. 15544034).

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Escherichia coli
Bottle
Autoclave
Coliforms
Room Temperature
• 2-YT Broth Powder (500 g)

Store at room temperature.

2-YT Broth (Yeast Extract Tryptone)
Dehydrated Culture Media
500 g
SDS
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Documents

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