Invitrogen™ 2-YT Broth
2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors.
Supplier: Invitrogen™ 22712020
2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors. The 2-YT formulation is similar to Lennox LB medium, but contains double the concentration of yeast extract and a 60% higher concentration of peptone. 2-YT medium allows cells to reach higher density and increase recombinant protein production compared to LB media formulations.
2-YT Broth formulation
Formulation per liter: 16 g SELECT Peptone 140 (pancreatic digest of casein), 10 g SELECT Yeast Extract, and 5 g NaCl.
Note: To prepare 1 L of 2-YT medium, weigh 31 g of powder and add distilled water to 1 L. Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 20 minutes at 121°C.
• Growth of hosts for replication of M13 vectors
• Recombinant protein expression
• Achieve higher plasmid DNA yield
Find the media format that fits you needs
We offer a variety of ready-to-use media formulations including LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301) and S.O.C Recovery Medium (Cat. No. 15544034).
Shipping Condition: Room Temperature
|• 2-YT Broth Powder (500 g)
Store at room temperature.
|2-YT Broth (Yeast Extract Tryptone)|
|Dehydrated Culture Media|
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok
For Research Use Only. Not for use in diagnostic procedures.