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Invitrogen™ Bac-to-Bac™ Baculovirus Expression System
Description
Includes
Each Bac-to-Bac Baculovirus Expression System includes 10μg of pFastBac1 vector, 0.5mL MAX Efficiency DH10Bac Chemically Competent Cells, pFastBac 1-Gus control vector, and 1mL of Cellfectin Reagent.
- Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
- Time-saving expression bacmid: expression cassette of pFastBacvector recombines with parent bacmid in DH10BacE. coli
- Competent Cells to form expression bacmid
- Bacmid is then transfected into insect cells for production of recombinant baculovirus particles
- Easy colony screening
- Parent bacmid in DH10BacE. colicontains segment of lacZα gene
- lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants
- Flexibility
- Comes with pFastBac1 vector that offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
- Two other vectors are offered separately
- pFastBacHT vector for production of histidine-tagged recombinant protein and pFastBacDual vector for expression of two proteins simultaneously
- Rapid purification of recombinant proteins
- Optional pFastBacHT vector produces histidine-tagged recombinant protein for rapid protein purification with nickel-chelating resin
Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | Polyhedrin |
| Product Type | Baculovirus Expression System |
| Content And Storage | Each Bac-to-Bac Baculovirus Expression System includes: • pFastBac1 vector (10 μg), store at -20°C • pFastBac 1-Gus control vector (4 ng), store at -20°C • MAX Efficiency DH10Bac Chemically Competent E. coli (1 kit with 5 x 0.1 mL), store at -80°C • ExpiFectamine Sf Transfection Reagent (1 mL), store at 4°C, do not freeze |
| Protein Tag | Untagged |
| Cloning Method | Restriction Enzyme/MCS |
| Quantity | 1 kit |
| Vector | pFastBac |
| Product Line | Bac-to-Bac™ |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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