Invitrogen™ Bac-to-Bac™ Baculovirus Expression System

Enables efficent production of recombinant baculovirus for expression testing in insect cells

Manufacturer:  Invitrogen™ 10359016

Catalog No. 10-359-016

Includes: Each Bac-to-Bac Baculovirus Expression System includes 10μg of pFastBac1 vector, 0.5mL MAX Efficiency DH10Bac Chemically Competent Cells, pFastBac 1-Gus control vector, and 1mL of Cellfectin Reagent.

Description

• Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
• Time-saving expression bacmid: expression cassette of pFastBacvector recombines with parent bacmid in DH10BacE. coli
• Competent Cells to form expression bacmid
• Bacmid is then transfected into insect cells for production of recombinant baculovirus particles
• Easy colony screening
• Parent bacmid in DH10BacE. colicontains segment of lacZα gene
• lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants
• Flexibility
• Comes with pFastBac1 vector that offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
• Two other vectors are offered separately
• pFastBacHT vector for production of histidine-tagged recombinant protein and pFastBacDual vector for expression of two proteins simultaneously
• Rapid purification of recombinant proteins
• Optional pFastBacHT vector produces histidine-tagged recombinant protein for rapid protein purification with nickel-chelating resin

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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Specifications

• Promoter: Polyhedrin
• Content And Storage: Each Bac-to-Bac Baculovirus Expression System includes:
  • pFastBac1 vector (10 µg), store at -20°C
  • pFastBac 1-Gus control vector (4 ng), store at -20°C
  • MAX Efficiency DH10Bac Chemically Competent E. coli (1 kit with 5 x 0.1 mL), store at -80°C
  • ExpiFectamine Sf Transfection Reagent (1 mL), store at 4°C, do not freeze
  
  
• Cloning Method: Restriction Enzyme/MCS
• Product Line: Bac-to-Bac™

• Product Type: Baculovirus Expression System
• Protein Tag: Untagged
• Vector: pFastBac

For Research Use Only. Not for use in diagnostic procedures.