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Invitrogen™ Bac-to-Bac™ Baculovirus Expression System

Enables efficent production of recombinant baculovirus for expression testing in insect cells

Supplier:  Invitrogen™ 10359016


Catalog No. 10-359-016

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Includes: Each Bac-to-Bac Baculovirus Expression System includes 10μg of pFastBac1 vector, 0.5mL MAX Efficiency DH10Bac Chemically Competent Cells, pFastBac 1-Gus control vector, and 1mL of Cellfectin Reagent.



  • Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
  • Time-saving expression bacmid: expression cassette of pFastBacvector recombines with parent bacmid in DH10BacE. coli
  • Competent Cells to form expression bacmid
  • Bacmid is then transfected into insect cells for production of recombinant baculovirus particles
  • Easy colony screening
  • Parent bacmid in DH10BacE. colicontains segment of lacZα gene
  • lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants
  • Flexibility
  • Comes with pFastBac1 vector that offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
  • Two other vectors are offered separately
  • pFastBacHT vector for production of histidine-tagged recombinant protein and pFastBacDual vector for expression of two proteins simultaneously
  • Rapid purification of recombinant proteins
  • Optional pFastBacHT vector produces histidine-tagged recombinant protein for rapid protein purification with nickel-chelating resin

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis



Each Bac-to-Bac Baculovirus Expression System includes:
• pFastBac1 vector (10 µg), store at -20°C
• pFastBac 1-Gus control vector (4 ng), store at -20°C
• MAX Efficiency DH10Bac Chemically Competent E. coli (1 kit with 5 x 0.1 mL), store at -80°C
• ExpiFectamine Sf Transfection Reagent (1 mL), store at 4°C, do not freeze

Restriction Enzyme/MCS
Baculovirus Expression System


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For Research Use Only. Not for use in diagnostic procedures.