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Invitrogen™ Bac-to-Bac™ HT Vector Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Designed for use as part of Bac-to-Bac Baculovirus Expression System for expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli

Manufacturer:  Invitrogen™ 10584027

Catalog No. 10-584-027

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Includes: The Bac-to-Bac HT Vector Kit includes 10µg of pFastBac HT vector and pFastBac HT-CAT control vector.



  • Strong polyhedrin promoter for protein expression
  • Three reading frames for simplified cloning
  • N-terminal 6 x His tag for simple purification of recombinant fusion proteins
  • TEV protease cleavage site for removal of histidine tag following protein purification

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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The Bac-to-Bac™ HT Vector Kit includes 10 µg of pFastBac™ HT vector and pFastBac HT-CAT control vector.

Store the vectors at -20°C. All components are guaranteed stable for 6 months when properly stored.
Restriction Enzyme ⁄ MCS
TEV Protease Recognition Site
His Tag (6x)
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For Research Use Only. Not for use in diagnostic procedures.