Invitrogen™ Bac-to-Bac™ HT Vector Kit

Catalog No.	Quantity
10-584-027	1 kit

Catalog No. 10-584-027 Supplier Invitrogen™ Supplier No. 10584027

Description

Includes

The Bac-to-Bac HT Vector Kit includes 10μg of pFastBac HT vector and pFastBac HT-CAT control vector.

Designed for use as part of Bac-to-Bac Baculovirus Expression System for expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli

• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6 x His tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of histidine tag following protein purification

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: Polyhedrin
• Product Type: Vector Kit
• Content And Storage: The Bac-to-Bac™ HT Vector Kit includes 10 μg of pFastBac™ HT vector and pFastBac HT-CAT control vector.
  
  Store the vectors at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Cleavage: TEV Protease Recognition Site
• Protein Tag: His Tag (6x)
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 1 kit
• Vector: pFastBac
• Product Line: Bac-to-Bac

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.