Invitrogen™ Bac-to-Bac™ Vector Kit
Designed for fast, small-scale production of recombinant baculovirus
Manufacturer: Invitrogen™ 10360014
- Contains pFastBac™1 vector, as well expression control vector, intended for use as part of Bac-to-Bac Baculovirus Expression System, which enables efficent production of recombinant baculovirus for expression testing in insect cells
- Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
- pFastBac 1 vector provided by this kit offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
- Time-saving expression bacmid: expression cassette of pFastBac vector recombines with parent bacmid in DH10BacE. coli
- Competent Cells to form expression bacmid: bacmid is then transfected into insect cells for production of recombinant baculovirus particles
- Easy colony screening
- Parent bacmid in DH10Bac E. colicontains segment of lacZα gene
- lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants
Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
|Contains 10 µg of pFastBac™1 vector and pFastBac™ 1-Gus control vector.
Store at -20°C.
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For Research Use Only. Not for use in diagnostic procedures.