Invitrogen™ Bac-to-Bac™ Vector Kit

Description

Includes

10μg of pFastBac1 vector and pFastBac 1-Gus control vector.

Designed for fast, small-scale production of recombinant baculovirus

• Contains pFastBac™1 vector, as well expression control vector, intended for use as part of Bac-to-Bac Baculovirus Expression System, which enables efficent production of recombinant baculovirus for expression testing in insect cells
• Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
• pFastBac 1 vector provided by this kit offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
• Time-saving expression bacmid: expression cassette of pFastBac vector recombines with parent bacmid in DH10BacE. coli
• Competent Cells to form expression bacmid: bacmid is then transfected into insect cells for production of recombinant baculovirus particles
• Easy colony screening
• Parent bacmid in DH10Bac E. colicontains segment of lacZα gene
• lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: Polyhedrin
• Product Type: Vector Kit
• Content And Storage: Contains 10 μg of pFastBac™1 vector and pFastBac™ 1-Gus control vector.
  
  Store at -20°C.
• Protein Tag: Untagged
• Cloning Method: Restriction Enzyme/MCS
• Quantity: 1 kit
• Vector: pFastBac
• Product Line: Bac-to-Bac™

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.