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Invitrogen™ Bac-to-Bac™ Vector Kit

Designed for fast, small-scale production of recombinant baculovirus

Manufacturer:  Invitrogen™ 10360014

Encompass

Catalog No. 10-360-014


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Includes: 10μg of pFastBac1 vector and pFastBac 1-Gus control vector.

Description

Description

  • Contains pFastBac™1 vector, as well expression control vector, intended for use as part of Bac-to-Bac Baculovirus Expression System, which enables efficent production of recombinant baculovirus for expression testing in insect cells
  • Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
  • pFastBac 1 vector provided by this kit offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
  • Time-saving expression bacmid: expression cassette of pFastBac vector recombines with parent bacmid in DH10BacE. coli
  • Competent Cells to form expression bacmid: bacmid is then transfected into insect cells for production of recombinant baculovirus particles
  • Easy colony screening
  • Parent bacmid in DH10Bac E. colicontains segment of lacZα gene
  • lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Specifications

Polyhedrin
Contains 10 µg of pFastBac™1 vector and pFastBac™ 1-Gus control vector.

Store at -20°C.
Restriction Enzyme/MCS
Bac-to-Bac™
Vector Kit
Untagged
pFastBac
SDS
Documents
Product Certifications
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For Research Use Only. Not for use in diagnostic procedures.