Invitrogen™ Bac-to-Bac™ Vector Kit  

Designed for fast, small-scale production of recombinant baculovirus

Manufacturer:  Invitrogen™ 10360014

Catalog No. 10-360-014

Includes: 10µg of pFastBac1 vector and pFastBac 1-Gus control vector.

Description

• Contains pFastBac™1 vector, as well expression control vector, intended for use as part of Bac-to-Bac Baculovirus Expression System, which enables efficent production of recombinant baculovirus for expression testing in insect cells
• Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
• pFastBac 1 vector provided by this kit offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
• Time-saving expression bacmid: expression cassette of pFastBac vector recombines with parent bacmid in DH10BacE. coli
• Competent Cells to form expression bacmid: bacmid is then transfected into insect cells for production of recombinant baculovirus particles
• Easy colony screening
• Parent bacmid in DH10Bac E. colicontains segment of lacZα gene
• lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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Specifications

• Vector: pFastBac
• Content And Storage: Contains 10 µg of pFastBac™1 vector and pFastBac™ 1-Gus control vector.
  
  Store at -20°C.
• Protein Tag: Untagged

• Promoter: Polyhedrin
• Product Line: Bac-to-Bac™
• Cloning Method: Restriction Enzyme ⁄ MCS

For Research Use Only. Not for use in diagnostic procedures.