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Invitrogen™ BaculoDirect™ C-Term Expression Kit
Description
Includes
All destination vectors are provided lyophilized and supercoiled.
Requires
Gateway™ entry clone, Gateway™ LR Clonase enzyme mix, and reaction buffer.
Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Promoter | Polyhedrin |
| Product Type | Expression Kit |
| Content And Storage | The BaculoDirect expression kits include five 0.3 µg vials of BaculoDirect Linear DNA, 125 µL of Cellfectin transfection reagent, frozen Sf9 cells, Gateway LR Clonase enzyme mix, GIBCO Unsupplemented Grace's Insect Medium, and ganciclovir. Store the BaculoDirect Linear DNA, Cellfectin reagent, and Grace's Medium at +4°C. Store LR Clonase mix at -80°C. Store Sf9 cells in liquid nitrogen. All components are guaranteed stable for 6 months if stored properly. |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | Gateway |
| Quantity | 5 transfections |
| Vector | BaculoDirect Vectors |
| Product Line | BaculoDirect™, Gateway™ |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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