Invitrogen™ BaculoDirect™ C-Term Transfection Kit

Catalog No.	Quantity
12-562-039	5 transfections

Catalog No. 12-562-039 Supplier Invitrogen™ Supplier No. 12562039

Description

Includes

Five 0.3μg vials of BaculoDirect Linear DNA, 125μL of Cellfectin transfection reagent, and ganciclovir.

Powerful and versatile eukaryotic system for high-level protein expression in insect cells

• Engineered to include attR sites for quick and efficient recombination with Gateway entry clone
• Gene of interest is recombined from entry clone into BaculoDirect Linear DNA using simple, one-hour LR reaction
• Resulting reaction mix contains recombinant baculovirus carrying gene of interest and is used directly to transfect insect cells
• Eliminates need for transforming bacteria and isolating large bacmid or co-transfection of transfer vector and linear baculovirus DNA into insect cells togreatly reduce hands-on time
• Purified baculovirus can be isolated in less than one week

• Gateway Linear DNA: Designed for rapid cloning with Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells
• Strong polyhedrin promoter for high-level expression
• R sites for efficient recombination with attL-flanked Gateway entry vector
• TK gene for negative selection using ganciclovir
• N-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA)
• TEV protease cleavage site for removal of V5-His tag following purification (BaculoDirect N-Term Linear DNA)
• C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA)

Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: Polyhedrin
• Product Type: Transfection Kit
• Content And Storage: The BaculoDirect™ transfection kits include five 0.3 μg vials of BaculoDirect Linear DNA, 125 μL Cellfectin transfection reagent, and ganciclovir.
  
  Store the BaculoDirect Linear DNA and Cellfectin at 4°C. All components are guaranteed stable for 6 months if stored properly.
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 5 transfections
• Vector: BaculoDirect Vectors
• Product Line: BaculoDirect, Gateway

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.