Invitrogen BioPrime Total FFPE Genomic Labeling System
Complete genomic DNA labeling kit designed for use in array comparative genomic hybridization applications (aCGH) using formalin-fixed paraffin-embedded (FFPE) samples
Manufacturer: Invitrogen A10965011
Previous protocols have required large amounts of very high quality DNA, which is often difficult to obtain from FFPE material. With a random prime amplification (RPA) method, using labeling mixes specifically formulated for FFPE samples, BioPrime Total FFPE Genomic Labeling System generates high yields of labeled DNA which is most representative of the starting sample. Increased call accuracy is achieved with higher yields of labeled material on the array, giving you more accurate calls.
This complete, all-inclusive kit is optimized to work across a wider range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allow for consistent, robust DNA yields of genomic FFPE DNA input. BioPrime Total for Agilent aCGH is supplied with Purelink purification which allows for low elution, eliminating the need for complicated volume-reduction steps. Purelink purification also reduces background due to dye bled found in other purification systems An optimized dye labeled nucleotide mix with new dNTP linkers, novel, application-specific Alexa Fluor 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. Complete kits are batch tested to assure quality in performance from lot to lot.
- Improved call rate with higher signal to noise ratios from Alexa Fluor 3 and 5 dyes
- Easy cleanup with PureLink purification eliminates the need for complicated volume-reduction steps
- Less channel bias due to enhanced reaction formulation for FFPE samples
- Lower requirements for input material using enzymatic RPA method
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|Alexa Fluor™ 3 FFPE 10X nucleotide mix and Alexa Fluor™ 5 FFPE 10X Nucleotide Mix, Exo-Klenow fragment (40U/μL), 5mM EDTA, 2.5X Random Primers solution, control DNA (Salmon Sperm), and DEPC-treated water, 10X Nucleotide Mixes, Purelink™ Genomic DNA purification columns and purification buffers.|
|Probes (Labeled DNA)|
|Alexa Fluor™ 3 FFPE 10X nucleotide mix and Alexa Fluor™ 5 FFPE 10X Nucleotide Mix, Exo-Klenow fragment, EDTA, Random Primers solution, control DNA (Salmon Sperm), and DEPC-treated water. Store at -80°C for long term storage. The 10X Nucleotide Mixes may be stored at 4°C for up to 4 weeks and should be protected from light. Also contains Purelink™ Genomic DNA purification columns and purification buffers. Store at room temperature.|
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For Research Use Only. Not for use in diagnostic procedures.