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Invitrogen™ BioPrime™ Total Genomic Labeling Module Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Supports a complete genomic DNA labeling system for use in array comparative genomic hybridization applications (aCGH) that allow for better call rates with precious samples

Manufacturer:  Invitrogen™ 18097012

Catalog No. 18-097-012


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Description

Description

The BioPrime Total Genomic Labeling Module is part of the system that offers users the following advantages:

  • Higher signal to noise with Alexa Fluor™ 3 and 5 dyes
  • Less channel bias due to novel reaction formulation
  • Widest range of input material (50ng - 3µg)
  • Simplified workflow with master mix formulation
This complete, all-inclusive kit is optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component-limited reaction allows for consistent, robust DNA yields of approx. 8µg with as little as 50ng of genomic DNA input. An optimized dye-labeled nucleotide mix with new dNTP linkers, novel application-specific Alexa Fluor 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. New master mix formulation streamlines reaction set-ups, increasing consistency. Complete kits are batch tested to assure quality in performance from lot to lot.
Contents and Storage:
The BioPrime™ Total Genomic Labeling Module contains Alexa Fluor 3 and Alexa Fluor 5 2x reaction mix, Exo-Klenow fragment (40 U/µL), 5mM EDTA, TE Buffer, and control DNA (Salmon Sperm). Available in a direct labeling format.

Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research

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Specifications

Specifications

Module, Genomic Labeling
Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research
30 reactions
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For Research Use Only. Not for use in diagnostic procedures. The BioPrime™ Total Genomic Labeling Module does not include Purelink™ Genomic DNA purification columns and purification buffers.