A CTP analog with biotin attached at the N 4 -position of the pyrimidine base by a 14-atom linker
Manufacturer: Invitrogen 19519016
Biotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP. The biotinylated RNA can be used as a probe in membrane-based or in situhybridizations. The amount of material provided is sufficient for up to 20 transcription reactions (1µg template).
Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC.
DNA and RNA Purification and Analysis, Nucleic Acid Labeling and Oligo Synthesis, RNA Labeling
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|Biotin-14-CTP is supplied at 10 mM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.|
|Biotin & Other Haptens|
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For Research Use Only. Not for use in diagnostic procedures.