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Invitrogen™ BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit
Description
Includes
The BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit contains the pLenti4/BLOCK-iT™-DEST vector, pLenti4-GW/H1/TO-lamin shRNA control vector, and One Shot™ Stbl3™ Chemically Competent E.Coli.
The BLOCK-iT Lentiviral RNAi Zeo Gateway Vector Kit contains the pLenti4/BLOCK-iT-DEST expression vector which enables lentiviral delivery and genomic integration of DNA coding for shRNA. Once expressed, the shRNA is processed by cellular machinery and initiates target-specific RNAi.
- Gateway Technology for efficient recombination of the RNAi cassette from the BLOCK-iT inducible pENTR™/H1/TO vector
- All required components for efficient lentiviral packaging, delivery, and integration of the shRNA
- Zeocin selection marker for fast selection of clonal cell lines containing the RNAi cassette
Lentiviral shRNA and miRNA, RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Virus-Based RNAi
Specifications
Specifications
| Product Type | RNAi Expression Vector Kit |
| Content And Storage | The BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit contains the pLenti4/BLOCK-iT™-DEST vector, pLenti4-GW/H1/TO-lamin shRNA control vector, and One Shot™ Stbl3™ Chemically Competent E.Coli. Store the pLenti4/BLOCK-iT™-DEST, pLenti6/TR vector, Blasticidin, ViraPower™ Lentiviral packaging mix, and the control vector at -20°C. Store the One Shot Stbl3™ Competent Cells 293 FT cell, and LR Clonase™ enzyme mix at -80°C. Store Lipofectamine™ 2000 at +4°C. All reagents are guaranteed stable for 6 months when properly stored. |
| Cloning Method | Gateway™ |
| Constitutive or Inducible System | Inducible |
| Delivery Type | Lentiviral |
| RNAi Type | shRNA |
| Selection Agent (Eukaryotic) | Zeocin™ |
| Vector | pLenti |
| Promoter | H1/TO |
| Format | Kit |
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For Research Use Only. Not for use in diagnostic procedures.
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