Invitrogen™ Bluo-Gal

Description

Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate.

Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate. As with X-gal, Bluo-Gal produces blue (lac+) bacterial colonies and blue (lac+) viral plaques. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.

Bluo-Gal can also be used as a substrate with the streptavidin-β-galactosidase conjugate in both immunoblot and immunocytochemistry assays. In the presence of β-galactosidase, Bluo-Gal yields a blue precipitate, which is insoluble in alcohols and xylenes. Bluo-Gal is soluble in dimethylformamide.

Primary applications
• Blue-white screening (deeper blue as compared to X-gal)—detect functional β-galactosidase (reporter gene expression)
• Histochemical detection of β-galactosidase activity in fixed tissues
• Bluo-Gal overlay assay

Find the media format that fits your needs
• Ready-to-use media formulations are available, such as LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301), or S.O.C recovery medium (Cat. No. 15544034).
• IPTG (Cat. No.15529019) is available for blue-white screening.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: • Bluo-Gal, substrate for β-galactosidase (1 g)
  
  Store at –5°C to –30°C.
  
  
• Description: Bluo-Gal, Cell-Permeant
• Detection Method: Colorimetric
• Format: Bottle
• Test Type: Blue/White Colony Screening, Immunoblotting, Immunocytochemical Assays
• Material: Halogenated Indolyl-β-galactoside Substrate
• Quantity: 1 g
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.