Invitrogen™ Calcium Phosphate Transfection Kit

Description

Includes

2 x 12mL Tissue Culture Sterile Water, 2 x 12mL 2X HEPES Buffered Saline (HBS), 3 x 1mL 2M CaCl₂, 20μg pcDNA™3.1/His/lacZ

Provides high-quality reagents to enable the introduction of DNA into eukaryotic cells via calcium phosphate co-precipitation

• Based on forming a calcium phosphate-DNA precipitate
• Facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis
• First developed by Graham and van der Ebb and was later modified by Wigle
• Used to transfect a wide variety of cell types for transient expression or for producing stable transformants
• DNA is mixed directly with a concentrated solution of CaCl₂, which is then added dropwise to a phosphate buffer to form a fine precipitate
• Aeration of the phosphate buffer while adding the DNA-CaCl₂ solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently
• Product size: 75 reactions

Cell Culture, Plasmid Transfection, Transfection

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: 2 × 12 ml Tissue Culture Sterile Water
  2 × 12 ml 2X HEPES Buffered Saline (HBS)
  3 × 1 ml 2 M CaCl₂
  20 μg pcDNA™3.1/His/lacZ
  
  Store all components at -5 to -30°C.
• Cell Type: Established Cell Lines
• Format: 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
• Sample Type: Plasmid DNA
• Transfection Technique: Chemical Transfection
• For Use With (Application): Transfection
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Type: Transfection Kit
• Quantity: 75 Reactions
• Serum Compatible: No

For Research Use Only. Not for use in diagnostic procedures.