Invitrogen™ ChargeSwitch™ Forensic DNA Purification Kit

Catalog No.	No. of Reactions
CS11200	100 Preps
CS1120010	960 Preps

Catalog No. CS11200 Supplier Invitrogen™ Supplier No. CS11200

Description

Includes

ChargeSwitch Lysis Buffer (L13)
ChargeSwitch Magnetic Beads
Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
ChargeSwitch Purification Buffer (N5)
ChargeSwitch Wash Buffer (W12)
ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

ChargeSwitch™ Forensic DNA Purification Kit uses magnetic bead technology to allow for rapid and efficient purification of genomic DNA from a variety of forensic samples, including poor or environmentally degraded samples, and is fully validated for Single Tandem Repeat (STR) analysis.

ChargeSwitch™ Forensic DNA Purification Kit uses magnetic bead technology to allow for rapid and efficient purification of genomic DNA from a variety of forensic samples, including poor or environmentally degraded samples, and is fully validated for Single Tandem Repeat (STR) analysis.

• Optimized for higher-purity DNA extraction
• Validated for STR profiling on diverse forensic samples-set
• Compatible with high-throughput protocols

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.
  
  Components:
  • ChargeSwitch™ Lysis Buffer (L13)
  • ChargeSwitch™ Magnetic Beads
  • Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
  • ChargeSwitch™ Purification Buffer (N5)
  • ChargeSwitch™ Wash Buffer (W12)
  • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

• Format: Kit
• Isolation Technology: ChargeSwitch Chemistry
• Sample Type: Forensic Sample
• For Use With (Application): Forensic
• High-throughput Compatibility: High-throughput Compatible
• No. of Reactions: 100 Preps
• Product Line: ChargeSwitch
• Product Type: Forensic DNA Purification Kit
• Quantity: 100 Preps
• Shipping Condition: Room Temperature
• Target: Genomic DNA
• Test Time: 15 min.

Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

Do you offer any kits for forensic DNA purification?

Yes, we offer our ChargeSwitch Forensic DNA Purification Kit. Please read more about DNA extraction here (http://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/dna-extraction/genomic-dna-extraction/forensic-dna-extraction.html).

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.