Invitrogen™ ChargeSwitch™ gDNA Micro Tissue Kit

Catalog No.	Quantity
CS11203	50 Preps

Catalog No. CS11203 Supplier Invitrogen™ Supplier No. CS11203

Description

Requires

Use of a magnetic accessory.

Sample size mouse ear clips of 3-5 mg tissue with a typical yield up to 5 μg.

• Sample size mouse ear clips of 3-5 mg tissue; Typical yield up to 5 μg
• Ultra-sensitive purification from laser microdissected samples
• Flexibility of an overnight or rapid protocol
• Reliable PCR performance from a range of micro-tissue samples
• ChargeSwitch gDNA Micro Tissue Kit provides high levels of performance required to purify high quality DNA from the smallest laser microdissected tissue samples and mouse ear clips
• Avoids introduction of enzyme inhibitors, which can often limit downstream applications when using very low levels of DNA

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Store in refrigerator (2–8°C).
• Format: Kit
• Isolation Technology: ChargeSwitch Chemistry
• Sample Type: Tissue
• For Use With (Application): PCR, Southern Blotting, Sequencing, Cloning
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• No. of Reactions: 50 Preps
• Product Line: ChargeSwitch
• Product Type: gDNA Micro Tissue Kit
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Target: Genomic DNA

Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.