Invitrogen™ ChargeSwitch™ gDNA Mini Tissue Kit

Catalog No.	Quantity
CS11204	25 Preps

Catalog No. CS11204 Supplier Invitrogen™ Supplier No. CS11204

Description

Includes

• ChargeSwitch Lysis Buffer (L13)
• ChargeSwitch Lysis Buffer (L15)
• ChargeSwitch Magnetic Beads
• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch Purification Buffer (N5)
• ChargeSwitch Wash Buffer (W12)
• ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

The ChargeSwitch gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after sample preparation and lysis.

The ChargeSwitch gDNA Mini Tissue Kit uses magnetic bead-based technology to allow rapid and efficient isolation of genomic DNA from between 10-25 mg of tissue such as mouse tail, spleen, bone, and hair in less than 15 minutes after sample preparation and lysis.

• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
• High Quality, High Yield Plasmid DNA
• Compatible with MagnaRack™ Magnetic Rack
• Improved Performance in Demanding Downstream Applications

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  All components of the ChargeSwitch™ gDNA Tissue Kits are shipped at room temperature. Upon receipt, store the Proteinase K and RNase A at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.
  
  Components:
  • ChargeSwitch™ Lysis Buffer (L13)
  • ChargeSwitch™ Lysis Buffer (L15)
  • ChargeSwitch™ Magnetic Beads
  • Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl₂, 50% glycerol)
  • RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • ChargeSwitch™ Purification Buffer (N5)
  • ChargeSwitch™ Wash Buffer (W12)
  • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

• Format: Kit
• Isolation Technology: ChargeSwitch Chemistry
• Scale: Mini
• Sample Type: Tissue
• For Use With (Application): PCR, Southern Blotting, Sequencing, Cloning
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: ChargeSwitch
• Product Type: gDNA Mini Tissue Kit
• Quantity: 25 Preps
• Shipping Condition: Room Temperature
• Target: Genomic DNA
• Test Time: 15 min.

Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.