Invitrogen™ ChargeSwitch™ Lysis Buffer L13

Catalog No.	Quantity
CS11202	800 mL

Catalog No. CS11202 Supplier Invitrogen™ Supplier No. CS11202

Description

ChargeSwitch Forensic DNA Purification Kit is magnetic bead method designed to accommodate all forensic crime scene sample types for STR profiling.

• ChargeSwitch Forensic DNA Purification Kit is magnetic bead method designed to accommodate all forensic crime scene sample types for STR profiling
• Scalable for single-tube use or for automated extraction

Order Info

Shipping Condition: Room Temperature

Specifications

• Buffer: Lysis Buffer
• Content And Storage: All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.
• Format: Kit
• For Use With (Application): DNA Extraction
• Product Line: ChargeSwitch
• Product Type: Lysis Buffer L13
• Quantity: 800 mL
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.