Invitrogen™ ChargeSwitch™ Plasmid ER Mini Kit

Description

Includes

• ChargeSwitch Magnetic Beads (25 mg/mL) in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch Lysis Buffer (L9)
• ChargeSwitch Precipitation Buffer (N5)
• ChargeSwitch Wash Buffer (W11)
• ChargeSwitch Wash Buffer (W12)
• ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
• Endotoxin Reduction Reagent (D1; ETRR)

Requires

Magnetic rack

ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA.

ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA from 1-5 mL of fresh, overnight bacterial cultures without the use of chaotropic salts, organic solvents, or ethanol, to yield up to 20 μg of transfection-grade plasmid DNA in a total time of about 10 minutes after sample preparation.

• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
• High quality, high yield plasmid DNA
• Compatible with MagnaRack™ magnetic rack
• Reliable performance in variety of downstream applications

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  All components of the ChargeSwitch™ Plasmid ER (Endotoxin Reduced) Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Store Precipitation Buffer (N5) at 4°C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store the remaining components at room temperature. All components are guaranteed stable for 6 months when stored properly.
  
  Components:
  • ChargeSwitch™ Magnetic Beads (25 mg/mL) in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20
  • RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • ChargeSwitch™ Lysis Buffer (L9)
  • ChargeSwitch™ Precipitation Buffer (N5)
  • ChargeSwitch™ Wash Buffer (W11)
  • ChargeSwitch™ Wash Buffer (W12)
  • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
  • Endotoxin Reduction Reagent (D1; ETRR)

• Format: Kit
• Isolation Technology: ChargeSwitch Chemistry
• Scale: Mini
• Sample Type: Bacterial Culture
• Final Product Type: Plasmid DNA
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
• Product Line: ChargeSwitch™
• Product Type: Plasmid ER Mini Kit
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Target: Plasmid DNA
• Test Time: 10 min.

For Research Use Only. Not for use in diagnostic procedures.