Invitrogen™ ChargeSwitch™ Plasmid ER Mini Kit

Catalog No.	Quantity
CS10100	50 Preps

Catalog No. CS10100 Supplier Invitrogen™ Supplier No. CS10100

Description

Includes

• ChargeSwitch Magnetic Beads (25 mg/mL) in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch Lysis Buffer (L9)
• ChargeSwitch Precipitation Buffer (N5)
• ChargeSwitch Wash Buffer (W11)
• ChargeSwitch Wash Buffer (W12)
• ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
• Endotoxin Reduction Reagent (D1; ETRR)

Requires

Magnetic rack

ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA.

ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA from 1-5 mL of fresh, overnight bacterial cultures without the use of chaotropic salts, organic solvents, or ethanol, to yield up to 20 μg of transfection-grade plasmid DNA in a total time of about 10 minutes after sample preparation.

• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
• High quality, high yield plasmid DNA
• Compatible with MagnaRack™ magnetic rack
• Reliable performance in variety of downstream applications

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  All components of the ChargeSwitch™ Plasmid ER (Endotoxin Reduced) Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Store Precipitation Buffer (N5) at 4°C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store the remaining components at room temperature. All components are guaranteed stable for 6 months when stored properly.
  
  Components:
  • ChargeSwitch™ Magnetic Beads (25 mg/mL) in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20
  • RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • ChargeSwitch™ Lysis Buffer (L9)
  • ChargeSwitch™ Precipitation Buffer (N5)
  • ChargeSwitch™ Wash Buffer (W11)
  • ChargeSwitch™ Wash Buffer (W12)
  • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
  • Endotoxin Reduction Reagent (D1; ETRR)

• Format: Kit
• Isolation Technology: ChargeSwitch Chemistry
• Scale: Mini
• Sample Type: Bacterial Culture
• Final Product Type: Plasmid DNA
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
• Product Line: ChargeSwitch
• Product Type: Plasmid ER Mini Kit
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Target: Plasmid DNA
• Test Time: 10 min.

Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.