Invitrogen™ ChargeSwitch™ Plasmid Yeast Mini Kit

Description

Includes

• ChargeSwitch Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
• RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
• ChargeSwitch Resuspension Buffer (R4)
• ChargeSwitch Lysis Buffer (L9)
• ChargeSwitch Precipitation Buffer (N5)
• ChargeSwitch Wash Buffer (W11)
• ChargeSwitch Wash Buffer (W12)
• ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

The ChargeSwitch Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 mL of Saccharomyces cerevisiae grown in selective minimal media.

The ChargeSwitch Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 mL of Saccharomyces cerevisiae grown in selective minimal media. Data generated from transformation and PCR experiments using ChargeSwitch purified DNA shows improved performance versus DNA purified by other methods.

• Sample size 1 mL S. cerevisiae culture
• Typical yield up to 25 ng
• Increased transformation efficiency
• Reagents that avoid PCR inhibition
• Purifies DNA with improved downstream performance
• Includes all necessary reagents for number of preps Listed and requires use of magnetic accessory

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  All components of the ChargeSwitch™ Plasmid Yeast Mini Kit are shipped at room temperature. Upon receipt, store components as follows: Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4°C. Store Precipitation Buffer (N5) at 4°C to improve performance. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.
  
  Components:
  • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
  • RNase A (5 mg/mL in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
  • ChargeSwitch™ Resuspension Buffer (R4)
  • ChargeSwitch™ Lysis Buffer (L9)
  • ChargeSwitch™ Precipitation Buffer (N5)
  • ChargeSwitch™ Wash Buffer (W11)
  • ChargeSwitch™ Wash Buffer (W12)
  • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

• Format: Kit
• Isolation Technology: ChargeSwitch Chemistry
• Scale: Mini
• Sample Type: Yeast
• Final Product Type: Plasmid DNA
• For Use With (Application): PCR, Cloning, Southern Blotting, Sequencing, Transformation
• Green Features: Less hazardous, Fewer resources used and less waste
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• No. of Reactions: 50 Preps
• Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
• Product Line: ChargeSwitch™
• Product Type: Plasmid Yeast Mini Kit
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Target: Plasmid DNA

For Research Use Only. Not for use in diagnostic procedures.