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Invitrogen™ Competent Cell Sampler Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Includes two reactions of four chemically competent E. coli strains

Manufacturer:  Invitrogen™ A10469

Catalog No. A10469


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Includes: Competent Cell Sampler contains two transformations each of four strains of chemically competent cells in One Shot™ format.

Description

Description

  • One Shot™ Mach1™ T1R, (Cat. No. C862003), is the fastest-growing chemically competent strain currently available. Mach1™ T1R colonies are clearly visible within eight hours of plating your transformation. From an overnight colony, minipreps can be performed after four hours of growth.
  • Genotype: F- φ80(lacZ)ΔM15 ΔlacX74 hsdR(rκ–, mκ +) ΔrecA1398 endA1 tonA
    • One Shot™ OmniMAX 2 T1R cells, (Cat. No. C854003), are perfect for use in all cloning applications, including Gateway™ technology. They offer the highest transformation efficiency of any chemically competent cell in the One Shot™ packaging format.
  • Genotype: F' {proAB+ lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80(lacZ)ΔM15 Δ(lacZYA-argF) U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD
    • One Shot™ Stbl3, (Cat. No. C7373-03), reduce the frequency of unwanted homologous recombination and so are perfect for cloning unstable DNA.
  • Genotype: F- mcrΒ mrr hsdcS20 (rΒ-, mΒ-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR) xyl-5 λ- leu mtl-1
    • One Shot™ TOP10 cells, (Cat. No. C4040-03), are perfect for routine cloning and included in many TOPO™ cloning and expression kits.
  • Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endA1 nupG λ-

Fast Growth: Yes
Blue-White Screening: Yes
Cloning Unstable DNA: Suitable for cloning unstable DNA
Library Construction: Yes
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Contains F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): Yes

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Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Mach1™ , Stbl3™ , TOP10, OmniMAX™2
One Shot
E. coli
> 1x109
8 transformations
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