Invitrogen™ CT-GFP Fusion TOPO™ Expression Kit

Description

Includes

CT-GFP Fusion Expression kit contain two boxes: 1. TOPO cloning box with pcDNA 3.1/CT-GFP TOPO cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid.

Designed to allow fusion of protein of interest to Cycle 3 GFP protein

• Provide topoisomerase-I activated pcDNA3.1/CT-GFP-TOPO vector
• Taq-amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top
• Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells
• Expression is easily detected in living cells using fluorescence
• Addition, recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP), Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Promoter: CMV
• Product Type: TOPO Expression Kit
• Reporter Gene: GFP (Cycle 3)
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored
• Protein Tag: GFP (Cycle 3)
• Cloning Method: TOPO-TA
• Quantity: 20 reactions
• Vector: TOPO-TA Vectors
• Product Line: TOPO™
• For Use With (Application): Reporter Assays

For Research Use Only. Not for use in diagnostic procedures.