Invitrogen™ DES™-Inducible Kit with pCoBlast

Description

Includes

Each kit contains 20μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ, Calcium Phosphate Transfection Kit, forward and reverse primers (2μg each), frozen S2 cells, GIBCO Schneider's Drosophila Medium, and the appropriate selection agent.

Small size (3.5kb) to improve DNA yields and increase subcloning efficiency

• Provides expression vector pMT/V5-His and a Blasticidin (pCoBlast) selection
• Uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to culture medium
• C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
• To facilitate cloning, set of three vectors-A, B, and C-is provided: Each vector has multiple cloning site in different reading frame relative to coding sequence of C-terminal tag

Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: MT
• Product Type: Inducible Kit
• Content And Storage: Each DES™-Inducible Kit contains 20 μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO™ Schneider's Drosophila Medium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Protein Tag Position (to your gene): C-terminal
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 1 kit
• Vector: pMT
• Product Line: DES™

For Research Use Only. Not for use in diagnostic procedures.