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Invitrogen™ DES™-Inducible/Secreted Kit with pCoBlast
Description
Includes
The Inducible/Secreted Kits contains 20μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2μg each), GIBCO Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent.
- This vector offers inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride
- N-terminal signal sequence from insect BiP gene is provided to direct recombinant fusion protein through secretory pathway of S2 cells into culture medium
- pMT/BiP/V5-His vector offers small size (3.6kb) to improve DNA yields and increase subcloning efficiency
- C-terminal V5 epitope tag for rapid detection withAnti-V5 Antibody
- C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
- To facilitate cloning, set of three vectors-A, B, and C-is provided: Each vector has multiple cloning site in different reading frame relative to BiP signal sequence
Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Protein Expression in Insect Cells
Specifications
Specifications
| Promoter | MT |
| Product Type | Inducible/Secreted Kit |
| Content And Storage | The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored. |
| Protein Tag Position (to your gene) | C-terminal |
| Protein Tag | His Tag (6x), V5 Epitope Tag |
| Cloning Method | Gateway |
| Quantity | 1 kit |
| Vector | pMT |
| Product Line | DES™ |
For Research Use Only. Not for use in diagnostic procedures.
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