Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ DES™-Inducible/Secreted Kit with pCoBlast

Catalog No. K513001
Encompass_Preferred
Click to view available options
Quantity:
1 kit

Includes

The Inducible/Secreted Kits contains 20μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2μg each), GIBCO Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent.

Provides vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and Blasticidin (pCoBlast) selection

  • This vector offers inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride
  • N-terminal signal sequence from insect BiP gene is provided to direct recombinant fusion protein through secretory pathway of S2 cells into culture medium
  • pMT/BiP/V5-His vector offers small size (3.6kb) to improve DNA yields and increase subcloning efficiency
  • C-terminal V5 epitope tag for rapid detection withAnti-V5 Antibody
  • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
  • To facilitate cloning, set of three vectors-A, B, and C-is provided: Each vector has multiple cloning site in different reading frame relative to BiP signal sequence

Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Protein Expression in Insect Cells

TRUSTED_SUSTAINABILITY

Specifications

Promoter MT
Product Type Inducible/Secreted Kit
Content And Storage The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Protein Tag Position (to your gene) C-terminal
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Gateway
Quantity 1 kit
Vector pMT
Product Line DES™

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.