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Invitrogen DETACHaBEAD Dynabeads Regulatory CD4+/CD25+ T Cell Kit
Use kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express intracellular transcription factor Foxp3
Manufacturer: Invitrogen 11363D
The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.
- Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
- Treg cells can be expanded with Dynabeads CD3/CD28 Treg Expander and remain Foxp3+
- Treg cells can be used in any downstream applications (e.g., suppression assays)
- Methodology: The isolation procedure includes the following steps:
- Negative isolation of untouched human CD4+ T-cells from PBMC
- Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset
- Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD
- Starting sample: PBMC
- Capacity: Processes ~1x109 cells total
- Cell Type: T Cells (Regulatory)
- Isolation Method: Depletion, then positive isolation
- Output Viability: >95%
- Sample Type: PBMC
- Starting Material (Cell No.): 1x108 PBMCs per isolation
- Target Species: Human
Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation
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Shipping Condition: Room Temperature
|Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation|
|2mL Antibody Mix Human CD4, 10mL Depletion MyOne™ Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1x109 cells|
|1x108 PBMCs per isolation|
|Depletion, then positive isolation|
|2 x 5mL|
|Storage: 2°C to 8°C.|
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For Research Use Only. Not for use in diagnostic procedures.