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Invitrogen™ DETACHaBEAD™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Use kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express intracellular transcription factor Foxp3

Manufacturer:  Invitrogen™ 11363D

Catalog No. 11-363-D



Includes: 2mL Antibody Mix Human CD4, 10mL Depletion MyOne; Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1 x 109 cells

Description

Description

The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

  • Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
  • Treg cells can be expanded with Dynabeads CD3/CD28 Treg Expander and remain Foxp3+
  • Treg cells can be used in any downstream applications (e.g., suppression assays)
  • Methodology: The isolation procedure includes the following steps:
  • Negative isolation of untouched human CD4+ T-cells from PBMC
  • Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset
  • Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD
  • Starting sample: PBMC
  • Capacity: Processes ~1x109 cells total
  • Cell Type: T Cells (Regulatory)
  • Isolation Method: Depletion, then positive isolation
  • Output Viability: >95%
  • Sample Type: PBMC
  • Starting Material (Cell No.): 1x108 PBMCs per isolation
  • Target Species: Human

Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation

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Shipping Condition: Room Temperature

Specifications

Specifications

Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation
2mL Antibody Mix Human CD4, 10mL Depletion MyOne™ Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1x109 cells
>95%
Research Grade
PBMC
Human
1x108 PBMCs per isolation
Depletion, then positive isolation
2 x 5mL
Storage: 2°C to 8°C.
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For Research Use Only. Not for use in diagnostic procedures.