Invitrogen™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit

Description

Includes

2mL Antibody Mix Human CD4, 10mL Depletion MyOne; Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1 x 10⁹ cells

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3.

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain
Foxp3+.
• Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology
The isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™

Starting sample
PBMC

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: T Cells (Regulatory)
• Content And Storage: Each Kit Contains:
  • 2 mL Antibody Mix Human CD4
  • 10 mL Depletion MyOne™ Dynabeads™
  • 5 mL Dynabeads™ CD25
  • 2 mL DETACHaBEAD™.
  
  These reagents are sufficient to process ∼1 x 10⁹ cells. Store components at 2°C to 8°C.
• Product Type: Cell Isolation Kit
• Isolation Technology: Depletion, then positive isolation
• No. of Cells: Processes ∼1 x 10⁹ cells total
• Output Viability: >95%
• Product Line: DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
• Purity or Quality Grade: Research Grade
• Quantity: 2 x 5 mL
• Sample Type: PBMC
• Shipping Condition: Room Temperature
• Starting Material Cell No.: 1 x 10⁸ PBMCs per isolation
• Target Species: Human

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.