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Invitrogen™ Exosome-Human CD63 Isolation/Detection Reagent (from cell culture media)
Description
Exosome-Human CD63 Isolation/Detection Reagent enables exosome (also called extracellular vesicles and multi-vesicular bodies) detection using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent.
- Obtain highly pure CD63+ exosomes
- View sample during handling
- Easily scalable protocol
- Detect exosomes via flow cytometry, in less than 1 hour
- Utilizes Dynabeads magnetic separation technology
- Allows easy purification of pre-enriched CD63+ exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting
- Bead-bound exosomes can be easily visualized by flow cytometry
- Monodispersed and relatively large Dynabeads (4.5 μm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour
- Magnetic handling also allows sample to be visualized due to the light brown color of beads
- Magnets allow for easy separation and purification
- Sample and bead volume can be easily scaled up or down according to your sample size or downstream applications
Good Mixing is Critical
- For successful exosome isolation, it is important to use a mixer that tilts and rotates to ensure that beads do not settle in tube
- Avoid end-over end rotation for small sample volumes (e.g., 100 μL)
- Please see user manual below for more guidelines
Specifications
Specifications
| Cell Type | Cells in culture |
| Content And Storage | Store at 2°C to 8°C. |
| Product Type | Isolation/Detection Reagent |
| Product Line | Dynabeads™ |
| Quantity | 3 mL |
| Sample Type | Cell Culture Supernatants |
| Target Species | Human |
| Diameter (Metric) | 4.5 μm |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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