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Invitrogen™ Exosome-Human CD9 Isolation Reagent (from cell culture) Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Enables isolation of CD9-positive extracellular vesicles such as exosomes from enriched cell culture media

Manufacturer:  Invitrogen™ 10614D

Catalog No. 10-614-D

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Obtain a highly pure exosome subset with easy protocol scalability with Exosome-Human CD9 Isolation Reagent (from cell culture). Following isolation, the specific sub-population of exosomes, ECVs can be further studied using methods such as Western blotting, qPCR, or next generation sequencing.

  • Minimal hands-on time for specific exosome isolation
  • Compatible with most downstream analysis
  • Recent studies suggest that exosome/ECV population consists of several subsets, possibly with different functions
  • Using immuno-affinity based Dynabeads magnetic separation technology, these subsets can be selectively isolated and studied
  • Cell culture media must first be enriched for exosomes
  • Can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation
  • Following enrichment, specific exosomes are isolated by binding to surface of magnetic beads
  • In addition to their high-quality sensitivity, reproducibility, and stability, Dynabeads allows you to see samples during handling due to color of magnetic beads
  • When a sample tube is placed in the magnet, bead-bound exosomes are pulled to side of tube, allowing for easy separation and buffer changes
  • Sample and bead volume can be easily scaled according to your sample size or downstream applications

Mix thoroughly to ensure that magnetic beads do not settle in the sample tube. A mixer which provides both tilting and rotation for optimal mixing is recommended.

Cell Analysis, Exosome Isolation, Exosomes

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Store in refrigerator (2–8°C).
Cell Cultures
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