Invitrogen™ CELLection™ Epithelial Enrich Dynabeads™

Description

Includes

5mL CELLection; Epithelial Enrich Dynabeads coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCam). 2 vials Releasing Buffer Component 1 (DNase I) and 2mL Relasing Buffer Component 2 are also supplied. Up to 2x10⁹ cells can be processed with this kit.

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC.

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC. Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.

High enrichment of viable human tumor cells
• Isolate directly from whole blood, bone marrow or PBMC
• Viable cells for any cellular applications

Methodology: Add CELLection™ Dynabeads™ to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection™ Dynabeads™ contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)

Note: If you want to deplete or use the isolated tumor cells for molecular applications, please use the equivalent product Dynabeads™ Epithelial Enrich (Cat. No. 161-02). This product contains the same antibody, but with no release of the cells from the beads after positive isolation.

Starting Samples: Whole blood, bone marrow, buffy coat, PBMC.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: Tumor Cells, Epithelial Cells
• Content And Storage: Each Kit Contains:
  • 5 mL CELLection™ Epithelial Enrich Dynabeads™ coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCam)
  • 2 vials Releasing Buffer Component 1 (DNase I)
  • 2 mL Releasing Buffer Component 2
  
  Up to 2 x 10⁹ cells can be processed with this kit. Store components at 2°C to 8°C.
• Product Type: Epithelial Enrichment Kit
• Isolation Technology: Positive Isolation
• No. of Cells: Processes ∼4 x 10⁹ cells total
• Output Viability: >95%
• Product Line: CELLection™, DYNAL™, Dynabeads™
• Purity or Quality Grade: Research Grade
• Quantity: 5 mL
• Reactivity: Human
• Sample Type: PBMC, Blood
• Shipping Condition: Room Temperature
• Starting Material Cell No.: 2 x 10⁷ PBMCs per isolation
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.